Biocytin tracer
WebBiocytin is a conjugate of biotin and lysine which is small and soluble. It is widely used in neuroanatomical research as a neuroanatomical tracer. Uses and applications. Biocytin …
Biocytin tracer
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WebMar 1, 1994 · Discussion This study has demonstrated that biocytin, an an- terograde and retrograde tract-tracer (King et al., 1989; Izzo, 1991; Pican~o-Diniz et al., 1992), is … WebAF 594 biocytin is a cell-impermeant fixable polar tracer. It consists of AF 594 dye, biotin, and aldehyde-fixable primary amine moieties. AF 594 is a bright, photostable red fluorophore with spectral characteristics similar to Texas Red (absorption max. at 586 nm, emission max. at 613 nm).
WebBiocytin ( (+)-Biocytin, Biotinyl-L-lysine) is a conjugate of D-biotin and L-lysine. Biocytin is an attractive neuronal tracer. It labels neurons fast over long distances, and it can be … WebJun 12, 2015 · TMR Biocytin is an attractive neuronal tracer. It labels neurons fast over long distances, and it can be used in conjunction with calcium dyes to report on neuronal activity in retrogradely ...
WebCF® Dye Biocytin. CF® Dye biocytin is a cell-impermeant, fixable polar tracer that is a conjugate of CF® Dye and biotin with an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell … WebNov 15, 2000 · Gap junctions serve many important roles in various tissues, but their abundance and diversity in neurons is only beginning to be understood. The tracer Neurobiotin has revealed many different networks interconnected by gap junctions in retina. We compared the relative permeabilities of five different retinal gap junctions by …
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WebTMR biocytin is a cell-impermeant, fixable polar tracer that combines the tetramethylrhodamine (TAMRA) with biotin and an aldehyde-fixable primary amine. Polar … reach fartherWebNov 15, 1993 · Subsequently, extracellular injections of the sensitive anterograde tracer biocytin were targeted into selected functional domains. Within the ocular dominance system, we found that long-range intrinsic connections tended to link the monocular regions of same-eye ocular dominance columns. Furthermore, we discovered that binocular … reach faqWebNEUROBIOTIN-Plus Tracer is a biotin label that is impervious to cleavage and breakdown by biotinidase. It is used for visualizing neural architecture and for the identification of gap junction coupling. Features. Fixable primary amine. Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells. how to spread positivityWebSome applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. ... Moreover, these small dextrans diffuse at rates equivalent to those of smaller tracers such as sulforhodamine 101 and biocytin (~2 millimeters/hour at 22°C) and about twice as fast as 10,000 MW dextrans. ... reach fashion studio softwareWebJan 1, 1992 · The properties of Neurobiotin™ as a tracer were further examined in this study by making pressure injections into different regions of the cerebral cortex or the rostral neostriatum of rats or by incubating striatal cells in culture with the tracer. ... refer to Reiner et al. (2000) and Luo et al. (2001). Biocytin and neurobiotin are similar ... reach fastighetsserviceWebOther Alexa Fluor Derivatives. To allow amplification of signals, especially in the finer processes of dye-filled neurons, we also offer Alexa Fluor 488 biocytin (), Alexa Fluor 546 biocytin and Alexa Fluor 594 biocytin ().These unique probes combine our Alexa Fluor 488, Alexa Fluor 546 and Alexa Fluor 594 fluorophores with biotin and an aldehyde … reach fashion studio software free downloadWeb576-19-2. Molecular weight. 372.48. Biocytin is invaluable as a rapidly transporting, sensitive neuronal tracer requiring little permeabilizing agents. It is an especially versatile marker for neuroanatomical investigations. Biocytin may be injected into brain by iontophoresis or by pressure injection methods, and localized in tissue sections ... reach fashion